Fusion Software from S-Matrix® Corporation



Learning statistical experimental design and data modelling theories normally requires a lot of time and effort from the layman. However, without profound knowledge about this, conventional statistical software cannot be used correctly and effectively and errors are inevitable. In everyday business life, however, it is usually not possible to acquire the required depth and expertise on one's own and a statistician must be consulted for such tasks.


With the technology of S-Matrix, software solutions are offered by which even the statistical layman can create and evaluate DoE experiments by the click of a button.



Fusion QbD Software Platform - Lifecycle Software for the Development, Validation, and Transfer of Analytical Methods


Fusion QbD is a modular software platform providing bidirectional data exchange to various chromtography systems and complete CFR21 Part11 support.

The application of QbD principles is common in product development. Unique feature of the Fusion software is the configured application of these principles to the development of chromatographic methods. Traditionally, LC methods are mostly developed according to the OFAT (One Factor At a Time) principle. This approach is not only time consuming, but also suppresses the effect of interactions between the considered qualitative and quantitative effects. Only by examining all levels for all factors under consideration, one can gain a clear understanding of the contexts.

However, this approach would lead to immeasurably long series of experiments and is therefore hardly practicable. By applying Design of Experiments (DoE), these experiments can be reduced by a factor of 5 and still obtain a complete picture of all the effects of the considered chromatographic factors for the entire experimental region and get a quantitative statement for them.

cromingo e.k., Fusion, Empower, Trainer, Fusion QbD Box


Fusion QbD® is the only software for method development that uses the real and complete QbD package in line with ICH Q8(R2 ). All predictions are based on empirical models generated from experimental raw data, not theoretic "first principle" functions or in-house algorithms. This allows Fusion QbD to be applied to all separation methods: Chiral, HILIC, Ion Exchange, Size Exclusion, Normal Phase and, of course, Reverse Phase. Fusion QbD is also great for difficult sample matrix where classic baseline separation is not achieved.


Fusion QbD

Handout Fusion QbD
  • Application Moduls:


    LC Method Development (FMD)


    QbD aligned method development and robustness optimization software with built in compliance and automated LC experimentation.



    Analytical Method Validation (FMV)


    Regulatory compliant validation for small and large molecule API and product. Also used successfully for Non-LC method validation (e.g. CE, GC, Q-NMR).



    Product Development (FPD)


    The perfect QbD-aligned software for formulation and process development as well as for the development of non-LC methods - wizard-driven experimental design, automated analysis and modeling, integrated Monte Carlo robustness simulation, elegant effect visualization graphics and comprehensive reporting. Especially suitable for the development and investigation of sample preparation methods.



    Fusion Inhaler Testing (FIT)


    Create sampling plans, export and import data from your CDS via validated data exchange, calculate particle size distribution results, and generate reports according to USP 601, Ph.Eur. 2.9.18, and ISO 27427.

  • Data Exchange Moduls:


    Agilent ChemStation/OpenLAB Data Exchange module


    Agilent LC Systems, including the Infinity I and II LCs.



    Thermo Chromeleon Data Exchange module


    Agilent LC Systems, including the Infinity I and II LCs.


    Dionex LC Systems, including the UltiMate and Vanquish LCs



    Waters Empower Data Exchange module


    Agilent LC Systems, including the Infinity I and II LCs.


    Waters HPLC, UPLC, and UPC^2 SFC systems.



Fusion Pro Software - Pure DoE Software


Fusion Pro is an expert system standalone software which is completely aligned with Quality by Design principles, and designed for working scientists and engineers. The software provides a complete suite of Design of Experiments (DOE) technologies, including advanced data modeling and Monte Carlo simulation modeling capabilities, to support your R&D work within a simple workflow based work environment. Fusion Pro is the perfect Design of Experiments (DOE) software for formulation studies, Process Optimization, and Product Development, from initial screening through final optimization.


If you only need a DoE software without interface to a chromatography software and without regulatory framework, then Fusion Pro is the product of your choice. Fusion Pro is available as stand-alone software but also as a network solution.


Fusion Pro

Quotation Request


We would be pleased to make you an individual offer. The following information is required for this:


Fusion QbD or Fusion Pro?


Workstation or Network?


What chromatopgraphy system do you have?


Number of LC systems that should be supported


Number of users per application module


You can also use the request form:

Quotation Request Form Fusion

Fusion QbD® from S-Matrix® Corporation


Chromatography-centric QbD-Software for the Development, Validation, and Transfer of Analytical Methods



The Perfect Software Solution for Analytical Procedure Lifecycle Management


Full support of Part 11 compliance / Cross-platform data integrity / Meets all regulatory guidelines and requirements


cromingo e.k., Fusion, Empower, Trainer, Rs Map Response

The Fusion QbD Software Suite consists of four independant modules:


  • Fusion LC Method Development (FMD)


    Chemistry Screening


    Fast, Automated Screening of Multiple Column and Mobile Phase Chemistries


    Supports All Separation Modes – e.g. RPC / NPC / SEC / IEX / HILIC / Chiral / SFC



    cromingo e.k., Fusion, Empower, Trainer,

    PeakTracker ™ - Automated PDA&MS Spectra Based Peak Tracking


    Automates, optimizes and simplifies the use of PDA and MS data in LC and LC/MS method development. Complex challenges PeakTracker can address include:


    Auto-deconvolution of Co-eluted Peaks


    Two or More Peaks with Identical Mass


    Non-ionizing & Non-absorbing Compounds



    cromingo e.k., Fusion, Empower, Trainer, peaktracker rgb

    Optimization with Integrated Robustness


    Fully Characterizes Robustness Throughout Entire Experimental Region


    Establishes Robust Method Operable Design Region (MODR)


    Simultaneously Address All Your Suitability Requirements


    Regulatory Accepted Robustness Metrics – e.g. Cp, Cpk, Cpm, % RSD, …



    cromingo e.k., Fusion, Empower, Trainer, Optimization

    Forced Degradation Studies


    Full Automation Suppor


    Peak Tracking Across Deg. Samples


    Composite Chromatogram Modeling and Visualization




    Fusion QbD - FMD Features
  • Fusion Method Validation (FMV)


    Full Method Validation Experiment Suite


    Calculations and reporting meet all current FDA/ICH/USP validation guidances – including the new USP‹1210›!


    Can be used for LC and Non-LC methods (e.g. GC, CE, Q-NMR)!


    Automates LC method validation experiments on multiple instruments and CDS systems!


    Regulatory accepted validation for both Small & Large Molecules!


    Statistically rigorous and defensible robustness testing!


    Handles multiple compounds – creates complete reports for each!


    Shortens your LC method validation time by as much as 75%!




    Fusion QbD - FMV Features
  • Fusion Product Development (FPD)


    Quality by Design on a Plate!


    If you are wondering where to start when implementing Quality by Design in your organization, Fusion Product Development (FPD) is the answer you've been looking for. With automated experimental design selection, validated data exchange, sophisticated data analysis tools, and comprehensive reporting, FPD is the perfect place to start your QbD journey.




    Fusion QbD - FPD Features
  • Fusion Inhaler Testing (FIT)


    The only fully automated off-the-shelf software solution for in vitro inhaler testing!


    Can be used for all DUSA and Inhaler Testing work – ACI, MMI, MSLI, FSA, FSI, and NGI®!


    Automates LC work on multiple instruments and CDS systems!


    Part 11 support for regulatory compliance!


    Built-in workflow management system with secure templating!


    Reduces or eliminates labor intensive manual data and report review!


    Reduces analyst time by a minimum of 40%!




    Fusion QbD - FIT Features

More information


If you would like to get to know the product, you are welcome to contact cromingo for a demo appointment. But you are also welcome to contact us for individual questions or a discussion about this product. Extensive information about the product can also be found on the manufacturer's website of S-Matrix Corporation: smatrix.com


Demo Request

Fusion Pro® - The Pure DoE-Software from S-Matrix


Scientists and engineers normally cannot spend the enormous amount of time and effort to learn the abstract statistical experimental design and data modeling theories which need to be understood to use general statistics software correctly and effectively, and avoid the many mistakes common to non-statistician users.


With Fusion Pro you can do it yourself without being a statistician!


cromingo e.k., Fusion, Empower, Trainer, fusion pro

Fusion Pro is an expert system standalone software which is completely aligned with Quality by Design principles, and designed for working scientists and engineers. The software provides a complete suite of Design of Experiments (DOE) technologies, including advanced data modeling and Monte Carlo simulation modeling capabilities, to support your R&D work within a simple workflow based work environment. Fusion Pro is the perfect Design of Experiments (DOE) software for formulation studies, process optimization, and product development, from initial screening through final optimization.


If you only need a DoE software without interface to a chromatography software and without regulatory framework, then Fusion Pro is the product of your choice. Fusion Pro can be considered equivalent to other established statistics programs, but stands out due to its special automation technology. the software is available as stand-alone software but also as a network solution.



Design Wizard


Automated Mode selects the most efficient and defensible DOE design for your defined variables and phase of work. User Interactive Mode lets you choose from its extensive design library, but don’t worry, only designs that are statistically valid for your defined variables will be enabled.



Response Data Handlers


Descriptive Statistics Toolset automatically computes analysis ready statistical metrics from data consisting of multiple test repeats per experiment run (e.g. Mean, Variance, Std. Dev., RSD, and %RSD). Time Series Toolset automatically computes analysis ready statistical curve-fit and key time-point metrics (e.g. f1, f2, Weibull, Result at Time Point = X, and Time Point at Result = Y) from data consisting of results run at multiple time points per experiment run (e.g. Dissolution, Synthesis).



Analysis Wizard


Automated Mode automatically derives a statistically defensible model (equation) from your experiment results for each included response. User Interactive Mode gives you full command of the analysis model options and the model building approaches.



Visualization Graphics


Instantly create 2D Effects Graphs, 3D Response Surface Graphs, and 4D Trellis Graphs. A 4D Trellis is a series of graphs which can show the effects of 4 variables at once on each modeled response or the effects of two critical variables on multiple responses at once.



Monte Carlo Methods


Mathematically rigorous and correct implementation of Monte Carlo simulation to automatically generate and model key robustness metrics such as Cp and Cpk for all included responses. This powerful capability enables you to characterize and visualize the effects of all study variables on not only mean (average) performance for all included responses, but also their effects on robustness.



Design Space


You can create a variety of reports containing a single Overlay Graph or a Trellis series of Overlay Graphs depicting your final QbD Design Space. You can also automatically scribe (superimpose) the Proven Acceptable Ranges (PARs) of your study variables on these graphs.




Fusion Pro Brochure


Math Modeling Verifications

Price Information


Fusion Pro Workstation Platform: €1070,-

Fusion Pro Network Platform(incl. 5 Concurrent User Licenses): €4280,-


The license fee is a one-time fee, running costs can arise from an additional maintenance contract to be concluded. With the maintenance contract you will receive technical support and can receive regular software updates.



More information


If you would like to get to know the product, you can contact cromingo for a demo version. We will provide you with the software for a period of 45 days free of charge with full functionality. However, you are also welcome to contact us for individual questions or a discussion about this product. Extensive information about the product can also be found on the manufacturer's website of S-Matrix Corporation: smatrix.com




Demo Request

Services for Fusion QbD®

  • Training


    We are committed to providing comprehensive training for all users of the Fusion QbD® software product modules. This training is offered in the form of on-site and online sessions. Upon completion, participants will receive a certificate confirming the training content.



    On-site Product Training


    Cromingo offers on-site user training programs for Fusion QbD software modules as a convenient and cost-effective alternative for larger research groups. These programs can be tailored to meet specific requirements. Training includes experimental strategies, Design of Experiments (DOE), Data analysis, graphical visualization, and product and process optimization.




    Fusion QbD Training

    Online Product Training


    Web-based user training is also available for all Fusion QbD software modules. These highly interactive sessions, conducted by qualified S-Matrix personnel, are particularly useful for refresher training and to help new users become familiar with the operation and workflow of the various Fusion QbD software modules.

    Please contact us for further information about our training offer.




    Getting in contact
  • Support


    Free technical support is provided for the Fusion software for 12 months from the date of purchase. After that, we provide technical support under a Support and Maintenance Agreement (SMA), which is renewable annually. We offer remote support by phone, email and web.

  • Software Qualification


    Certified Software Qualification


    In cooperation with S-Matrix, two Fusion QbD software qualification options are offered:


    Independent qualification: The software qualification is performed by the customer with the S-Matrix QMD package (see below).


    On-site software qualification. Software qualification is performed by an S-Matrix certified representative on site.


    Qualification Master Documentation Package (QMD Package)



    Qualifizierungs-Master-Dokumentationspaket (QMD-Paket)


    We create a Software Qualification Master Documentation Package (QMD Package) for each release version of Fusion QbD. The QMD contains all documents, testing plans, and data sets required to fully qualify Fusion QbD software installation and operation in customer environments. The QMD covers:


    All 21 CFR Part 11 compliance support tools and functions.


    All installed Fusion Application Modules (FAMs - e.g. Fusion LC Method Development, Fusion LC Method Validation)


    All CDS Data Exchange Modules (DEMs) – e.g.:


    » Agilent ChemStation/OpenLab DEM

    » Thermo Scientific Chromeleon DEM

    » Waters Empower DEM


    We also maintain a complete documentation set to support customer software qualification:


    21 CFR 11 Support Assessment Report


    GAMP 5 Classification Document


    Risk Assessment Document


    Deployment Diagrams


    Detailed Installation/Setup Guides


    URS Documents for each module

  • Software Demo


    Please contact us if you would like to have the software presented. We would be happy to provide you with the software for a trial phase and support you in evaluating the software.




    Demo Request

Frequently Asked Questions about Fusion QbD


General Aspects

  • What is the difference between the Fusion QbD approach for developing analytical methods compared to other tools?


    Whereas the technology of other LC method development tools is primarily based on historical theories, like the solvophobic one, Fusion uses a statistical approach based on the currently investigated set of data. That's also why Fusion can be applied to any chromatography type.

  • As the technology is based on a statistical approach, how much statistical knowledge is required for working with the software?


    Fusion is a solution that provides DoE designs and statistical modelling to be applied by non-statisticians. There is only the need to interpret the calculated outcome correctly. However, for users having the required expertise Fusion still offers a user defined mode for setting up the designs and perform data modelling.

Process & Procedure

  • How many injections are needed for a complete development study?


    The length of a design setup depends on several factors such as the number of numeric and categorical study factors, the investigated ranges and the stage of the development phase. Therefore, this can not be answered in general. However, as Fusion calculates specific equations and data models for every analytical response from scratch more injections are required for screening and optimization experiments compared to an approach where the equations (i.e. a chemical theory) already exist. Thus, with the obtained outcome one benefits from data models that a more specific and precise for any desired peak response , not exclusively for retention data.

  • Which pH range do you recommend to start with during a first screening. Would it be sufficient to use one acidic and one basic pH value?


    You should not mix basic and acidic pH values during a study, as these are two different chemistry systems. In which of these regions to perform the study should be investigated in advance. You could either select one acidic and one basic pH value and define these buffers as a buffer type variable or you could run several pH values preferably using the OLP mode to investigate, which pH region would work best for your compounds. During screening you should then use pH as a variable with up to 6 pH values and a range of about 1 pH unit.

  • How is the predictive value of the Fusion results. Are there still manual adjustments required to obtain a final method? What is the experience?


    We strongly dissuade from changing settings manually without the use of the software as there is a risk of compromising the already determined design space. Any refinements the user would like to perform should be performed by using the software.

  • Can predicted results be visualized in Fusion?


    Yes, from the calculated models simulation chromatograms can be displayed taking into account elution time, peak shape and peak resolution. In combination with the peak tracking feature peak names can clearly be assigned to the simulated chromatograms.

  • How does Fusion take different column dimensions into account?


    Fusion hasn't yet implemented a feature to scale a method to different column dimensions and operating conditions that give equivalent chromatographic performance. However, there are quite a lot of these tools on the market offered for free by vendors for chromatographic systems and analytical separation columns.

  • Is there a restriction with regard to the number of compounds Fusion is able to manage during an experiment?


    No. We can demonstrate examples with more than 90 compounds.

  • Can gradient steps be defined?


    Yes, up to 3 steps can be defined. However, we recommend to avoid multiple gradient steps, as with each step a further robustness issue is implemented into the method. Fusion has already proved to develop single step gradient methods with adequate baseline separation and acceptable run times in multiple customer studies.

  • Can Fusion perform peak tracking?


    With the latest release Fusion provides a patented peak tracking technology, that performs hyperaccurate peak tracking by the click on a button. Fusion uses 3D PDA spectral data augmented with standard UV peak results data to automatically identify each peak in each experiment chromatogram. Fusion will also fully utilize 3D mass spectral data for experiments run on LC systems configured with the Waters Acquity QDa Mass Detector (QDa).

  • Can Fusion handle large molecules where baseline separation is hardly achieved?


    As any peak response can be analyzed also not baseline separated peaks can be evaluated using responses such as e.g. retention time difference or p/v ratios.

  • Are methods developed with Fusion prepared for method transfer?


    By defining the permitted and expected variation of all relevant study factors with Fusion a possible method transfer can already been respected during method development. We recommend to include an initial isocratic hold time in the pump gradient and study this as a variable method parameter for simulating the dwell volume of different HPLC systems.

  • How does Fusion determine robustness for the final method?


    Fusion QbD determines a multivariate design space, that also includes robustness. So robustness is not investigated after the determination of a design space only for a single point, but already proven for the entire design space from the obtained data models. This can be reached by running a monte carlo simulation using the calculated models and applying process capability metrics (Cp, Cpk, Cpm, …). This is our understanding of a design space.

Implementation

  • How is data exchange between Fusion and a CDS implemented?


    Fusion offers an automated bidirectional data exchange with Empower, Chromeleon and ChemStation. Fusion does not directly access the database instances in Empower or Chromeleon, but uses the native CDS interfaces for creating ready-to-run sequences and methods during data export and reading out predefined chromatogram results during import. For this purpose, the Fusion software needs to be installed on a CDS client computer.

  • Can Fusion be used in an IT network?


    Yes, Fusion is network capable also in a Citrix environment.

  • Can Fusion be used in a GxP environment?


    Yes, Fusion is prepared for part 11 compliance. A user management with access groups and different user roles can be defined, all actions can be tracked by an audit trail, e-signature workflows can be configured and data security is also given by the use of encrypted database files and protection of the files that can only be read out in the context of a defined project container.

Instrument Support

  • What instrumentation do you recommend when using Fusion?


    Fusion works best with a UPLC equipped with a quaternary pump and having column and eluent switching capabilities. With these conditions various mobile and stationary phases including several pH settings can be studied in a single sequence. The online preparation mode for buffer strength, pH, additive and salt concentration eliminates the need to prepare a single eluent solution for each desired condition by building the proportioning required for each experiment run into the instrument methods it automatically constructs.

  • Which instruments are supported for the control by Fusion?


    Fusion offers instrument control support for a the most commonly used LC systems in pharmaceutical industry and the range of supported instruments increases from year to year. Currently supported instruments are listed here. Instrument control means, that the required instrument methods can be created automatically in the respective CDS through the Fusion data exchange functionality.

  • Which instruments are supported for the control by Fusion?


    Basically yes, methods for GC, CE or non-Fusion supported LC systems can also be developed. With Fusion Product Development, you can freely compile your design and carry out the data exchange in the same way with the CDS. This already saves a lot of time and provides data security. The exported designs alone would still have to be edited manually after the export by preparing the required instrument methods and adding the necessary equilibration and conditioning steps. There is also no possibility of visualizing chromatograms and generating so-called trend responses, i.e. parameters based on the number of peaks. In addition to Fusion's own modeling technology, the decisive advantage over other DoE products lies in the ability to exchange audited data with a CDS.

Purchase

  • Can you give any references for success stories with Fusion?


    We have collected some show cases in these presentations:




    Customer Testimonials


    Fusion QbD - LC Method Development - Protein and Peptide Examples
  • What would be the price of investment?


    The price depends on whether you decide for a standalone or network solution. Beside that there is a license model based on application modules, user and system licenses. Please contact us directly to discuss you individual requirements and situation in order to prepare an individual quote for you using our:




    Quotation Request Form